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Appl Environ Microbiol. 2011 Feb;77(3):1118-22. doi: 10.1128/AEM.01733-10. Epub 2010 Dec 10.

mathFISH, a web tool that uses thermodynamics-based mathematical models for in silico evaluation of oligonucleotide probes for fluorescence in situ hybridization.

Author information

1
Department of Civil and Environmental Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706-1691, USA. yilmaz@cae.wisc.edu

Abstract

Mathematical models of RNA-targeted fluorescence in situ hybridization (FISH) for perfectly matched and mismatched probe/target pairs are organized and automated in web-based mathFISH (http://mathfish.cee.wisc.edu). Offering the users up-to-date knowledge of hybridization thermodynamics within a theoretical framework, mathFISH is expected to maximize the probability of success during oligonucleotide probe design.

PMID:
21148691
PMCID:
PMC3028703
DOI:
10.1128/AEM.01733-10
[Indexed for MEDLINE]
Free PMC Article
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