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Science. 2011 Jan 21;331(6015):304-8. doi: 10.1126/science.1199082. Epub 2010 Dec 9.

Direct conversion of C. elegans germ cells into specific neuron types.

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1
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York, NY 10032, USA. bt2189@columbia.edu

Abstract

The ability of transcription factors to directly reprogram the identity of cell types is usually restricted and is defined by cellular context. Through the ectopic expression of single Caenorhabditis elegans transcription factors, we found that the identity of mitotic germ cells can be directly converted into that of specific neuron types: glutamatergic, cholinergic, or GABAergic. This reprogramming event requires the removal of the histone chaperone LIN-53 (RbAp46/48 in humans), a component of several histone remodeling and modifying complexes, and this removal can be mimicked by chemical inhibition of histone deacetylases. Our findings illustrate the ability of germ cells to be directly converted into individual, terminally differentiated neuron types and demonstrate that a specific chromatin factor provides a barrier for cellular reprogramming.

PMID:
21148348
PMCID:
PMC3250927
DOI:
10.1126/science.1199082
[Indexed for MEDLINE]
Free PMC Article

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