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Protein Expr Purif. 2011 Apr;76(2):238-47. doi: 10.1016/j.pep.2010.12.003. Epub 2010 Dec 10.

Purify First: rapid expression and purification of proteins from XMRV.

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1
Protein Expression Laboratory, SAIC-Frederick Inc., NCI-Frederick, Frederick, MD 21702, USA.

Abstract

Purifying proteins from recombinant sources is often difficult, time-consuming, and costly. We have recently instituted a series of improvements in our protein purification pipeline that allows much more accurate choice of expression host and conditions and purification protocols. The key elements are parallel cloning, small scale parallel expression and lysate preparation, and small scale parallel protein purification. Compared to analyzing expression data only, results from multiple small scale protein purifications predict success at scale-up with greatly improved reliability. Using these new procedures we purified eight of nine proteins from xenotropic murine leukemia virus-related virus (XMRV) on the first attempt at large scale.

PMID:
21146612
DOI:
10.1016/j.pep.2010.12.003
[Indexed for MEDLINE]
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