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Bioorg Med Chem Lett. 2011 May 1;21(9):2756-8. doi: 10.1016/j.bmcl.2010.11.028. Epub 2010 Nov 12.

Potentiation of ribonuclease cytotoxicity by a poly(amidoamine) dendrimer.

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1
Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA.

Abstract

Variants of bovine pancreatic ribonuclease (RNase A) engineered to evade the endogenous ribonuclease inhibitor protein (RI) are toxic to human cancer cells. Increasing the basicity of these variants facilitates their entry into the cytosol and thus increases their cytotoxicity. The installation of additional positive charge also has the deleterious consequence of decreasing ribonucleolytic activity or conformational stability. Here, we report that the same benefit can be availed by co-treating cells with a cationic dendrimer. We find that adding the generation 2 poly(amidoamine) dendrimer in trans increases the cytotoxicity of RI-evasive RNase A variants without decreasing their activity or stability. The increased cytotoxicity is not due to increased RI-evasion or cellular internalization, but likely results from improved translocation into the cytosol after endocytosis. These data indicate that co-treatment with highly cationic molecules could enhance the efficacy of ribonucleases as chemotherapeutic agents.

PMID:
21144746
PMCID:
PMC3081967
DOI:
10.1016/j.bmcl.2010.11.028
[Indexed for MEDLINE]
Free PMC Article
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