Format

Send to

Choose Destination
J Mol Biol. 1990 Jun 20;213(4):613-5.

Crystallization and preliminary X-ray studies of the VL domain of the antibody McPC603 produced in Escherichia coli.

Author information

1
Genzentrum, Universit├Ąt, Martinsried, F.R.G.

Abstract

The VL domain, obtained from a recombinant Fv fragment of the antibody McPC603 expressed in Escherichia coli, has been crystallized as a dimer from 2 M-(NH4)2SO4 (pH 4.0). The crystals are hexagonal, space group P6(1)22. The cell dimensions are a = b = 86.48 A, c = 76.64 A, with a VL monomer as the asymmetric unit. The crystals diffract to 2.0 A. The structure was solved by Patterson search using the VL domain of the Fab fragment of McPC603 and the VL dimer REI.

PMID:
2113586
DOI:
10.1016/S0022-2836(05)80247-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center