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Genetics. 2011 Feb;187(2):623-31. doi: 10.1534/genetics.110.124362. Epub 2010 Dec 6.

Plasticity in the purine-thiamine metabolic network of Salmonella.

Author information

1
Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA.

Abstract

In Salmonella enterica, 5-aminoimidazole ribonucleotide (AIR) is the precursor of the 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) pyrophosphate moiety of thiamine and the last intermediate in the common HMP/purine biosynthetic pathway. AIR is synthesized de novo via five reactions catalyzed by the purF, -D, -T, -G, and -I gene products. In vivo genetic analysis demonstrated that in the absence of these gene products AIR can be generated if (i) methionine and lysine are in the growth medium, (ii) PurC is functional, and (iii) 5-amino-4-imidazolecarboxamide ribotide (AICAR) has accumulated. This study provides evidence that the five steps of the common HMP/purine biosynthetic pathway can be bypassed in the synthesis of AIR and thus demonstrates that thiamine synthesis can be uncoupled from the early purine biosynthetic pathway in bacteria.

PMID:
21135073
PMCID:
PMC3030501
DOI:
10.1534/genetics.110.124362
[Indexed for MEDLINE]
Free PMC Article

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