Format

Send to

Choose Destination
Virol J. 2010 Dec 6;7:361. doi: 10.1186/1743-422X-7-361.

Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection.

Author information

1
Grupo de Epidemiologia y Salud Poblacional (GESP) Escuela de Salud Publica, Facultad de Salud, Universidad del Valle, Cali, Colombia Calle 4b 36-140, Cali-Colombia. lyda1oso@gmail.com

Abstract

BACKGROUND:

We compared the diagnostic accuracy and reproducibility of commercially available NS1-based dengue tests and explored factors influencing their sensitivities.

METHODS:

Paired analysis of 310 samples previously characterized as positive (n = 218) and negative (n = 92) for viral isolation and/or RT-PCR and/or IgM seroconversion. Masked samples were tested by two observers with Platelia™ Dengue NS1 Ag, second generation Pan-E™ Dengue Early ELISA, SD Dengue NS1 Ag ELISA, Dengue NS1 Ag STRIP™, and SD BIOLINE™ Dengue Duo (NS1/IgM/IgG).

RESULTS:

SD BIOLINE™ NS1/IgM/IgG had the highest sensitivity (80.7% 95%CI 75-85.7) with likelihood ratios of 7.4 (95%CI 4.1-13.8) and 0.21 (95%CI 0.16-0.28). The ELISA-format tests showed comparable sensitivities; all below 75%. STRIP™ and SD NS1 had even lower sensitivities (<65%). The sensitivities significantly decreased in samples taken after 3 days of fever onset, in secondary infections, viral serotypes 2 and 4, and severe dengue. Adding IgM or IgG to SD NS1 increased its sensitivity in all these situations.

CONCLUSIONS:

The simultaneous detection of NS1/IgM/IgG would be potentially useful for dengue diagnosis in both endemic and non endemic areas. A negative result does not rule out dengue. Further studies are required to assess the performance and impact of early laboratory diagnosis of dengue in the routine clinical setting.

PMID:
21134275
PMCID:
PMC3016282
DOI:
10.1186/1743-422X-7-361
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center