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Protein Expr Purif. 2011 May;77(1):104-11. doi: 10.1016/j.pep.2010.11.016. Epub 2010 Dec 2.

Screening of genetic parameters for soluble protein expression in Escherichia coli.

Author information

1
The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. erik.vernet@cpr.ku.dk

Abstract

Soluble expression of proteins in a relevant form for functional and structural investigations still often remains a challenge. Although many biochemical factors are known to affect solubility, a thorough investigation of yield-limiting factors is normally not feasible in high-throughput efforts. Here we present a screening strategy for expression of biomedically relevant proteins in Escherichia coli using a panel of six different genetic variations. These include engineered strains for rare codon supplementation, increased disulfide bond formation in the cytoplasm and novel vectors for secretion to the periplasm or culture medium. Combining these variants with expression construct truncations design, we report on parallel cloning and expression of more than 300 constructs representing 24 selected proteins; including full-length variants of human growth factors, interleukins and growth factor binding proteins. This rapid screening approach appears highly suitable for high-throughput efforts targeting either large sets of proteins or more focused investigations regarding individual high-profile targets.

PMID:
21130169
DOI:
10.1016/j.pep.2010.11.016
[Indexed for MEDLINE]

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