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J Pediatr Surg. 2010 Dec;45(12):2305-11. doi: 10.1016/j.jpedsurg.2010.08.022.

Identification of neuroblastoma stem cells by characterization of side population cells in the human neuroblastoma SK-N-SH cell line.

Author information

1
Department of Surgery, Children's Hospital of Fudan University, Shanghai 201102, China.

Abstract

PURPOSE:

The purpose of the study was to sort side population (SP) cells from the neuroblastoma SK-N-SH cell line and to systematically investigate whether this population has stem cell characteristics.

METHODS:

Side population and non-SP cells were separated from the SK-N-SH cell line by flow cytometry, and their morphologic characteristics were analyzed by light and electron microscopy. We also used Western blotting to analyze marker proteins, Cell Counting Kit-8 assay for proliferative ability, series differentiation studies for differentiation properties, and Matrigel invasion study and tumorigenicity assay for malignant potential.

RESULTS:

The SK-N-SH SP cells expressed high levels of stem cell markers, had high proliferative and malignant abilities, and had the capacity for rapid differentiation. The non-SP cells expressed differentiated cell marker proteins at high levels, had low proliferative and malignant abilities, and exhibited slow differentiation.

CONCLUSIONS:

The SK-N-SH SP cells have cancer stem cell-like properties.

PMID:
21129535
DOI:
10.1016/j.jpedsurg.2010.08.022
[Indexed for MEDLINE]

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