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Opt Lett. 2010 Dec 1;35(23):4018-20. doi: 10.1364/OL.35.004018.

Simultaneous dual molecular contrasts provided by the absorbed photons in photoacoustic microscopy.

Author information

1
Department of Ophthalmology, Keck School of Medicine, University of Southern California, 1450 San Pablo Street, Los Angeles, California 90033, USA.

Abstract

We investigated the feasibility of simultaneously imaging two distinctive molecular contrasts provided by the absorbed photons in biological tissues with a single light source. The molecular contrasts are based on two physical effects induced by the absorbed photons: photoacoustics (PA) and autofluorescence (AF). In an integrated multimodal imaging system, the PA and AF signals were detected by a high-sensitivity ultrasonic transducer and an avalanche photodetector, respectively. The system was tested by imaging ocular tissue samples, including the retinal pigment epithelium and the ciliary body. The acquired images provided information on the spatial distributions of melanin and lipofuscin in these samples.

PMID:
21124598
PMCID:
PMC3293242
DOI:
10.1364/OL.35.004018
[Indexed for MEDLINE]
Free PMC Article

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