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J Cosmet Dermatol. 2010 Dec;9(4):267-75. doi: 10.1111/j.1473-2165.2010.00520.x.

Evaluation of apoptosis regulatory markers in androgenetic alopecia.

Author information

1
Department of Dermatology, Faculty of Medicine, Al-Minya University, Al-Minya, Egypt. moetazeldomyati@yahoo.com

Abstract

BACKGROUND:

Androgenetic alopecia (AGA) is a common androgen-induced progressive disorder; the pathways of which are regulated by local genetic codes and hormonal control. Meanwhile, it is unclear whether an altered proliferation or increased apoptosis could contribute to its pathogenesis.

AIMS:

To evaluate the role of some apoptosis regulatory markers and follicular proliferation in the pathogenesis of AGA.

PATIENTS/METHODS:

Thirty biopsies were taken from the frontal (bald) area and occipital (hair-bearing) area of 15 male patients with AGA, as well as five specimens from the frontal area of five age-matched controls. The biopsies were stained with apoptosis regulatory markers (Bcl-2, p53, Bax & Fas) and PCNA (proliferating cell nuclear antigen), as well as TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling) staining for the detection of DNA fragmentation in apoptotic cells.

RESULTS:

Bcl-2 expression was localized to epidermal basal layer and follicular dermal papilla with highly significant correlation with PCNA expression (P < 0.001). Perifollicular lymphocytic infiltrate of the bald area showed significant expression of Bcl-2. However, pro-apoptotic Bax and Fas were expressed in the epidermis and not in the hair follicles which does not show any apoptotic keratinocytes by TUNEL staining.

CONCLUSION:

The low proliferation rate in the bald area of patients, together with persistent perifollicular inflammatory infiltrate as evidenced by the anti-apoptotic Bcl-2 expression in dermal lymphocytes, would result in follicular miniaturization and fibrosis.

[Indexed for MEDLINE]

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