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Mamm Genome. 2010 Dec;21(11-12):550-5. doi: 10.1007/s00335-010-9303-5. Epub 2010 Nov 27.

Multiple loci contribute to genome-wide recombination levels in male mice.

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1
School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA. brenda_murdoch@wsu.edu

Abstract

Recent linkage-based studies in humans suggest the presence of loci that affect either genome-wide recombination rates, utilization of recombination hotspots, or both. We have been interested in utilizing cytological methodology to directly assess recombination in mammalian meiocytes and to identify recombination-associated loci. In the present report we summarize studies in which we combined a cytological assay of recombination in mouse pachytene spermatocytes with QTL analyses to identify loci that contribute to genome-wide levels of recombination in male meiosis. Specifically, we analyzed MLH1 foci, a marker of crossovers, in 194 F2 male mice derived from a subspecific cross between CAST/EiJ and C57BL/6J parental strains. We then used these data to uncover loci associated with individual variation in mean MLH1 values. We identified seven recombination-associated loci across the genome (on chromosomes 2, 3, 4, 14, 15, 17, and X), indicating that there are multiple recombination "setting" loci in mammalian male meiosis.

PMID:
21113599
PMCID:
PMC3002158
DOI:
10.1007/s00335-010-9303-5
[Indexed for MEDLINE]
Free PMC Article
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