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Cell. 2010 Nov 24;143(5):774-88. doi: 10.1016/j.cell.2010.11.007.

Mechanisms determining the morphology of the peripheral ER.

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1
Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.

Abstract

The endoplasmic reticulum (ER) consists of the nuclear envelope and a peripheral network of tubules and membrane sheets. The tubules are shaped by the curvature-stabilizing proteins reticulons and DP1/Yop1p, but how the sheets are formed is unclear. Here, we identify several sheet-enriched membrane proteins in the mammalian ER, including proteins that translocate and modify newly synthesized polypeptides, as well as coiled-coil membrane proteins that are highly upregulated in cells with proliferated ER sheets, all of which are localized by membrane-bound polysomes. These results indicate that sheets and tubules correspond to rough and smooth ER, respectively. One of the coiled-coil proteins, Climp63, serves as a "luminal ER spacer" and forms sheets when overexpressed. More universally, however, sheet formation appears to involve the reticulons and DP1/Yop1p, which localize to sheet edges and whose abundance determines the ratio of sheets to tubules. These proteins may generate sheets by stabilizing the high curvature of edges.

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PMID:
21111237
PMCID:
PMC3008339
DOI:
10.1016/j.cell.2010.11.007
[Indexed for MEDLINE]
Free PMC Article

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