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Antiviral Res. 2011 Jan;89(1):98-108. doi: 10.1016/j.antiviral.2010.11.012. Epub 2010 Nov 23.

Pentagalloylglucose downregulates cofilin1 and inhibits HSV-1 infection.

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1
Biomedicine Research and Development Center of Jinan University, Guangzhou Huangpu Road West 601, Guangzhou, Guangdong 510632, China.

Abstract

To investigate the anti-herpesvirus mechanism of pentagalloylglucose (PGG), we compared the proteomic changes between herpes simplex virus type 1 (HSV-1) infected MRC-5 cells with or without PGG-treatment, and between non-infected MRC-5 cells with or without PGG-treatment by 2-DE and MS-based analysis. Differentially expressed cellular proteins were mainly involved with actin cytoskeleton regulation. Significantly, PGG can down-regulate cofilin1, a key regulator of actin cytoskeleton dynamics. PGG can inhibit HSV-1-induced rearrangements of actin cytoskeleton which is important for infectivity. Furthermore, cofilin1 knockdown by siRNA also inhibited the HSV-1-induced actin-skeleton rearrangements. Both PGG-treatment and cofilin1 knockdown can reduce HSV-1 DNA, mRNA, protein synthesis and virus yields. Altogether, the results suggested that down-regulating cofilin1 plays a role in PGG inhibiting HSV-1 infection. PGG may be a promising anti-herpesvirus agent for drug development.

PMID:
21108969
DOI:
10.1016/j.antiviral.2010.11.012
[Indexed for MEDLINE]

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