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J Virol. 2011 Feb;85(4):1871-4. doi: 10.1128/JVI.01663-10. Epub 2010 Nov 24.

Seeing the portal in herpes simplex virus type 1 B capsids.

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1
Structural Computational Biology and Molecular Biophysics Program, Baylor College of Medicine, Houston, Texas 77030, USA.

Abstract

Resolving the nonicosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase-contrast electron cryomicroscopy to enhance the image contrast of ice-embedded herpes simplex virus type 1 capsids. Image reconstruction enabled us to retrieve the structure of the unique portal vertex in the context of the icosahedral capsid and, for the first time, show the subunit organization of a portal in a virus infecting eukaryotes. Our map unequivocally resolves the 12-subunit portal situated beneath one of the pentameric vertices, thus removing uncertainty over the location and stoichiometry of the herpesvirus portal.

PMID:
21106752
PMCID:
PMC3028901
DOI:
10.1128/JVI.01663-10
[Indexed for MEDLINE]
Free PMC Article
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