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Curr Microbiol. 2011 Mar;62(3):1023-7. doi: 10.1007/s00284-010-9818-8. Epub 2010 Nov 21.

Rapid quantification of lipids in Acremonium chrysogenum using Oil red O.

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  • 1Department of Chemical and Biological Engineering, Korea University, 1-5Ka, Anam-Dong, Sungbuk-Ku, Seoul, 136-701, Republic of Korea.

Abstract

A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml(-1) of lipids in A. chrysogenum.

PMID:
21104083
DOI:
10.1007/s00284-010-9818-8
[PubMed - indexed for MEDLINE]
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