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Nat Biotechnol. 2010 Dec;28(12):1287-9. doi: 10.1038/nbt.1708. Epub 2010 Nov 21.

A robust system for production of minicircle DNA vectors.

Author information

1
Department of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, California, USA. markay@stanford.edu

Abstract

Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues. To improve minicircle production, we genetically modified Escherichia coli to construct a producer strain that stably expresses a set of inducible minicircle-assembly enzymes, ΦC31 integrase and I-SceI homing endonuclease. This bacterial strain produces purified minicircles in a time frame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minicircles in place of plasmids in mammalian transgene expression studies.

PMID:
21102455
PMCID:
PMC4144359
DOI:
10.1038/nbt.1708
[Indexed for MEDLINE]
Free PMC Article

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