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FEMS Microbiol Lett. 1990 Jan 1;54(1-3):263-9.

Interspecies electro-transformation in Corynebacteria.

Author information

1
Université Paris-Sud, Centre d'Orsay, Institut de Génétique et Microbiologie, France.

Abstract

Plasmid DNA was efficiently electro-transformed into intact cells of nine Corynebacteria strains belonging to Brevibacterium lactofermentum, Brevibacterium flavum, Corynebacterium glutamicum and Corynebacterium melassecola. Relationships were explored between transformation efficiency and parameters such as electric field strength and pulse length, DNA concentration, physiological state and concentration of the cells. In optimal conditions, more than 10(7) transformants per microgram of DNA could be obtained. Electro-transformation with plasmid DNA isolated from different sources indicates that DNA modification may play a role in transformation efficiency.

PMID:
2108897
DOI:
10.1016/0378-1097(90)90294-z
[Indexed for MEDLINE]

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