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Rev Soc Bras Med Trop. 2010 Sep-Oct;43(5):575-9.

[Identification by PCR and antifungal susceptibility of vaginal clinical Candida sp isolates].

[Article in Portuguese]

Author information

1
Curso de Farmácia Bioquímica Clínica, Universidade Regional Integrada do Alto Uruguai e das Missões, Santo Ângelo, RS. izabelalmeida@hotmail.com

Abstract

INTRODUCTION:

Vaginal candidiasis is a condition that affects innumerous fertile women. Candida albicans is the most frequently isolated species from vaginal discharge; however, other different species that are more resistant to antifungal drugs can be identified in vaginal clinical samples.

METHODS:

The species of 30 vaginal Candida isolates was identified by PCR using the universal ITS4 primer and species-specific primers for C. albicans, C. glabrata, C. tropicalis and C. krusei. The sensitivity pattern to amphotericin B, fluconazole and voriconazole was assessed using the CLSI M27-A2 macrodilution method.

RESULTS:

The PCR assay revealed 28 C. albicans and 2 samples showed amplification for C. albicans and C. glabrata primers. The minimum inhibitory concentration for amphotericin B ranged from 0.03 µg/mL to 0.25 µg/mL, for fluconazole from 0.125 µg/ml to 16 µg/mL and for voriconazole from 0.03 µg/mL to 0.25 µg/m.

CONCLUSIONS:

Identification at Candida species level by PCR assay could be relevant for clinical management of these infections.

PMID:
21085873
[Indexed for MEDLINE]
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