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Methods Mol Biol. 2011;702:419-27. doi: 10.1007/978-1-61737-960-4_30.

The generation and the manipulation of human multipotent adipose-derived stem cells.

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Institut de Recherche, Signalisation, Biologie du Développement et Cancer, UMR6543 Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia-Antipolis, Nice, France.


In this chapter, we describe a method to isolate and to expand multipotent adipose-derived stem (hMADS) cells from human adipose tissue. We also describe culture conditions to differentiate them into adipocytes at a high rate. This culture system provides a powerful means for studying the first steps of human adipose cell development and a route for investigating effects of drugs on the biology of adipocytes. Finally, we provide a protocol to investigate gene function during proliferation and differentiation of hMADS cells by means of siRNA-mediated gene silencing approaches or forced expression by transducing hMADS cells permissive to infection with murine retrovirus vectors.

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