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Bioconjug Chem. 2010 Dec 15;21(12):2327-31. doi: 10.1021/bc100348q. Epub 2010 Nov 16.

Multiplexed and reiterative fluorescence labeling via DNA circuitry.

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1
Department of Bioengineering and Department of Chemistry, Rice University, Houston, Texas 77005, USA.

Abstract

A class of reactive DNA circuits was adapted as erasable molecular imaging probes that allow fluorescent reporting complexes to be assembled and disassembled on a biological specimen. Circuit reactions are sequence-dependent and therefore facilitate multiplexed (multicolor) detection. Yet, the ability to disassemble reporting complexes also allows fluorophores to be removed and new circuit complexes to be used to label additional markers. Thus, these probes present opportunities to increase the total number of molecular targets that can be visualized on a biological sample by allowing multiple rounds of fluorescence microscopy to be performed.

PMID:
21080622
PMCID:
PMC3002180
DOI:
10.1021/bc100348q
[Indexed for MEDLINE]
Free PMC Article
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