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Methods Mol Biol. 2011;693:143-62. doi: 10.1007/978-1-60761-974-1_9.

Transgenesis in mouse embryonic stem cells.

Author information

1
Department of Pediatrics and Adolescent Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA. vanree.janine@mayo.edu

Abstract

Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, -promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.

PMID:
21080279
DOI:
10.1007/978-1-60761-974-1_9
[Indexed for MEDLINE]

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