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Methods Mol Biol. 2011;693:57-73. doi: 10.1007/978-1-60761-974-1_4.

Cryopreservation of mouse spermatozoa and in vitro fertilization.

Author information

1
Division of Reproductive Engineering, Center for Animal Resources & Development (CARD), Kumamoto University, Kumamoto, Japan.

Abstract

Cryopreservation of mouse spermatozoa has become the foremost technique for preserving large numbers of different strains of mice with induced mutations. Recently, we have established procedures for cryopreservation of mouse spermatozoa and in vitro fertilization using cryopreserved spermatozoa to obtain a relatively high fertilization rate. This chapter attempts to show these procedures in simple terms.

PMID:
21080274
DOI:
10.1007/978-1-60761-974-1_4
[Indexed for MEDLINE]

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