Comparison of transcription profiles between pro-SP-C+/CD90+ cells and bone marrow-mesenchymal stem cells (BM-MSCs). (a) The area-proportional Venn diagram presenting the overlap between transcripts expressed in pro-SP-C+/CD90+ cells (blue) and BM-MSCs (red). (b) Functional annotation clustering in specifically expressed gene sets in pro-SP-C+/CD90+ cells (blue) or BM-MSCs (red). Original array data are available at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=zpapfyaqumsqmvw&acc=GSE21095. (c) Semiquantitative RT–PCR for eight genes identified through the microarray analysis. Forkhead box f1 (Foxf1), T-box 4 (Tbx4), FBJ murine osteosarcoma viral oncogene homolog B (FosB), and laminin α5, that were selected out of the annotations of ‘transcription' and/or ‘lung development', were highly expressed by pro-SP-C+/CD90+ cells. At the same time, distal-less homeobox 5 (DLX5), N-cadherin, homeobox C10 (HOXC10), and hyaluronan synthase 1 (HAS1), that were chosen from the annotation of ‘skeletal system development' or ‘cell adhesion', were highly expressed by BM-MSCs. Three bathes of pro-SP-C+/CD90+ cells and three batches of BM-MSCs were examined. β-Actin was used as an endogenous control. In RT–PCR for Foxf1, Tbx4, FosB, laminin α5, and β-actin, a representative negative control (pro-SP-C+/CD90+ cells batch1 without reverse transcriptase reaction) is shown as no-RT. In RT–PCR for DLX5, N-cadherin, HOXC10, and HAS1, a representative negative control (BM-MSCs batch1 without reverse transcriptase reaction) is shown as no-RT.