Send to

Choose Destination
See comment in PubMed Commons below
Anal Biochem. 2011 Mar 15;410(2):310-2. doi: 10.1016/j.ab.2010.11.015. Epub 2010 Nov 13.

Bicinchoninic acid (BCA) assay in low volume.

Author information

  • 1Department of Pathology and the Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.


The BCA assay is a colorimetric method for estimating protein concentration. In 96-well plates, the relationship between protein content and absorbance is nearly linear over a wide range; however, performance is reduced in lower volume. To overcome this limitation, we performed the BCA assays in opaque, white 384-well plates. These plates emit fluorescence between 450-600 nm when excited at 430 nm; thus, their fluorescence is quenched by the BCA chromophore (λ(max) 562 nm). This arrangement allowed accurate determination of protein content using only 2 μL of sample. Moreover, soluble flourescein could replace the white plates, creating a homogenous format.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center