Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2010 Dec;7(12):989-94. doi: 10.1038/nmeth.1532. Epub 2010 Nov 14.

A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.

Author information

1
Cell and Molecular Biology Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Abstract

To exploit the full potential of human pluripotent stem cells for regenerative medicine, developmental biology and drug discovery, defined culture conditions are needed. Media of known composition that maintain human embryonic stem (hES) cells have been developed, but finding chemically defined, robust substrata has proven difficult. We used an array of self-assembled monolayers to identify peptide surfaces that sustain pluripotent stem cell self-renewal. The effective substrates displayed heparin-binding peptides, which can interact with cell-surface glycosaminoglycans and could be used with a defined medium to culture hES cells for more than 3 months. The resulting cells maintained a normal karyotype and had high levels of pluripotency markers. The peptides supported growth of eight pluripotent cell lines on a variety of scaffolds. Our results indicate that synthetic substrates that recognize cell-surface glycans can facilitate the long-term culture of pluripotent stem cells.

PMID:
21076418
PMCID:
PMC2994976
DOI:
10.1038/nmeth.1532
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center