Format

Send to

Choose Destination
See comment in PubMed Commons below
J Lipid Res. 2011 Feb;52(2):272-7. doi: 10.1194/jlr.M012013. Epub 2010 Nov 12.

Unsupported planar lipid membranes formed from mycolic acids of Mycobacterium tuberculosis.

Author information

1
Department of Physics, University of Washington, Seattle, WA 351560, USA.

Abstract

The cell wall of mycobacteria includes a thick, robust, and highly impermeable outer membrane made from long-chain mycolic acids. These outer membranes form a primary layer of protection for mycobacteria and directly contribute to the virulence of diseases such as tuberculosis and leprosy. We have formed in vitro planar membranes using pure mycolic acids on circular apertures 20 to 90 μm in diameter. We find these membranes to be long lived and highly resistant to irreversible electroporation, demonstrating their general strength. Insertion of the outer membrane channel MspA into the membranes was observed indicating that the artificial mycolic acid membranes are suitable for controlled studies of the mycobacterial outer membrane and can be used in nanopore DNA translocation experiments.

PMID:
21076119
PMCID:
PMC3023547
DOI:
10.1194/jlr.M012013
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center