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Proc Natl Acad Sci U S A. 2010 Nov 30;107(48):20810-5. doi: 10.1073/pnas.1014840107. Epub 2010 Nov 12.

Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in large copy numbers.

Author information

1
Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA.

Abstract

Before secretory vesicles undergo exocytosis, they must recruit the proteins syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in the plasma membrane. GFP-labeled versions of both proteins cluster at sites where secretory granules have docked. Single-particle tracking shows that minority populations of both molecules are strongly hindered in their mobility, consistent with their confinement in nanodomains. We measured the fluorescence of granule-associated clusters, the fluorescence of single molecules, and the numbers of unlabeled syntaxin-1 and SNAP-25 molecules per cell. There was a more than 10-fold excess of SNAP-25 over syntaxin-1. Fifty to seventy copies each of syntaxin-1 and SNAP-25 molecules were associated with a single docked granule, many more than have been reported to be required for fusion.

PMID:
21076040
PMCID:
PMC2996426
DOI:
10.1073/pnas.1014840107
[Indexed for MEDLINE]
Free PMC Article

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