Format

Send to

Choose Destination
J Mol Biol. 2011 Jan 14;405(2):325-30. doi: 10.1016/j.jmb.2010.10.031. Epub 2010 Nov 10.

Structure of human complement C8, a precursor to membrane attack.

Author information

1
University of Oxford, Wellcome Trust Centre for Human Genetics, Oxford OX3 7BN, UK.

Abstract

Complement component C8 plays a pivotal role in the formation of the membrane attack complex (MAC), an important antibacterial immune effector. C8 initiates membrane penetration and coordinates MAC pore formation. High-resolution structures of C8 subunits have provided some insight into the function of the C8 heterotrimer; however, there is no structural information describing how the intersubunit organization facilitates MAC assembly. We have determined the structure of C8 by electron microscopy and fitted the C8α-MACPF (membrane attack complex/perforin)-C8γ co-crystal structure and a homology model for C8β-MACPF into the density. Here, we demonstrate that both the C8γ protrusion and the C8α-MACPF region that inserts into the membrane upon activation are accessible.

PMID:
21073882
PMCID:
PMC3021121
DOI:
10.1016/j.jmb.2010.10.031
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center