Transmembrane potential measurement with carbocyanine dye diS-C3-(5): fast fluorescence decay studies

M Síp; P Herman; J Plásek; V Hrouda

doi:10.1016/1011-1344(90)85037-w

Transmembrane potential measurement with carbocyanine dye diS-C3-(5): fast fluorescence decay studies

J Photochem Photobiol B. 1990 Jan;4(3):321-8. doi: 10.1016/1011-1344(90)85037-w.

Authors

M Síp¹, P Herman, J Plásek, V Hrouda

Affiliation

¹ Institute of Physics, Charles University, Prague, Czechoslovakia.

PMID: 2107293
DOI: 10.1016/1011-1344(90)85037-w

Abstract

The mechanism of carbocyanine dye diS-C3-(5) fluorescence intensity variations with transmembrane potential changes has been studied using time-resolved fluorescence spectroscopy. Clear evidence is given of the transmembrane-potential-dependent partition of the dye among various sites with different fluorescence lifetimes. It was found that fluorescence decay profiles reflect the transmembrane potential changes.

MeSH terms

Benzothiazoles
Calibration
Carbocyanines*
Diffusion
Fluorescent Dyes*
Humans
Membrane Potentials*
Potassium / physiology
Quinolines*
Spectrometry, Fluorescence
Time Factors
Tumor Cells, Cultured

Substances

Benzothiazoles
Carbocyanines
Fluorescent Dyes
Quinolines
3,3'-dipropyl-2,2'-thiadicarbocyanine
Potassium