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EMBO Rep. 2010 Dec;11(12):977-84. doi: 10.1038/embor.2010.171. Epub 2010 Nov 12.

Uncovering new substrates for Aurora A kinase.

Author information

1
Department of Cell and Developmental Biology, Centre for Genomic Regulation, Barcelona, Spain.

Abstract

Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.

PMID:
21072059
PMCID:
PMC2999863
DOI:
10.1038/embor.2010.171
[Indexed for MEDLINE]
Free PMC Article
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