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Science. 2010 Nov 12;330(6006):985-9. doi: 10.1126/science.1196554.

Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124.

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1
Department of Medicine, Hematology Division, Stanford University, Stanford, CA 94305, USA.

Abstract

The orphan G protein-coupled receptor (GPCR) GPR124/tumor endothelial marker 5 is highly expressed in central nervous system (CNS) endothelium. Here, we show that complete null or endothelial-specific GPR124 deletion resulted in embryonic lethality from CNS-specific angiogenesis arrest in forebrain and neural tube. Conversely, GPR124 overexpression throughout all adult vascular beds produced CNS-specific hyperproliferative vascular malformations. In vivo, GPR124 functioned cell-autonomously in endothelium to regulate sprouting, migration, and developmental expression of the blood-brain barrier marker Glut1, whereas in vitro, GPR124 mediated Cdc42-dependent directional migration to forebrain-derived, vascular endothelial growth factor-independent cues. Our results demonstrate CNS-specific angiogenesis regulation by an endothelial receptor and illuminate functions of the poorly understood adhesion GPCR subfamily. Further, the functional tropism of GPR124 marks this receptor as a therapeutic target for CNS-related vascular pathologies.

PMID:
21071672
PMCID:
PMC3099479
DOI:
10.1126/science.1196554
[Indexed for MEDLINE]
Free PMC Article

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