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PLoS One. 2010 Oct 29;5(10):e13741. doi: 10.1371/journal.pone.0013741.

The mouse blood-brain barrier transcriptome: a new resource for understanding the development and function of brain endothelial cells.

Author information

1
Department of Anatomy, University of California San Francisco, San Francisco, California, United States of America. richard.daneman@ucsf.edu

Abstract

The blood-brain barrier (BBB) maintains brain homeostasis and limits the entry of toxins and pathogens into the brain. Despite its importance, little is known about the molecular mechanisms regulating the development and function of this crucial barrier. In this study we have developed methods to highly purify and gene profile endothelial cells from different tissues, and by comparing the transcriptional profile of brain endothelial cells with those purified from the liver and lung, we have generated a comprehensive resource of transcripts that are enriched in the BBB forming endothelial cells of the brain. Through this comparison we have identified novel tight junction proteins, transporters, metabolic enzymes, signaling components, and unknown transcripts whose expression is enriched in central nervous system (CNS) endothelial cells. This analysis has identified that RXRalpha signaling cascade is specifically enriched at the BBB, implicating this pathway in regulating this vital barrier. This dataset provides a resource for understanding CNS endothelial cells and their interaction with neural and hematogenous cells.

PMID:
21060791
PMCID:
PMC2966423
DOI:
10.1371/journal.pone.0013741
[Indexed for MEDLINE]
Free PMC Article

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