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Methods Mol Biol. 2011;682:37-48. doi: 10.1007/978-1-60327-409-8_4.

In situ labeling of DNA breaks and apoptosis by T7 DNA polymerase.

Author information

1
Departments of Neurosurgery and Molecular & Cellular Biology, Baylor College of Medicine, and Michael E DeBakey VA Medical Center, Houston, TX, USA. vdidenko@bcm.edu

Abstract

The native T7 DNA polymerase is a fast and highly processive enzyme that can be used for in situ detection of apoptosis and various types of DNA breaks. The technique is quick and simple, and was shown to label earlier stages of apoptosis compared to the terminal transferase technique. The in situ labeling applications of T7 DNA polymerase are presented and summarized from the DNA damage detection standpoint. The detailed protocols are provided together with the discussion of their advantages and limitations.

PMID:
21057919
PMCID:
PMC3324588
DOI:
10.1007/978-1-60327-409-8_4
[Indexed for MEDLINE]
Free PMC Article

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