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Zhonghua Yan Ke Za Zhi. 2010 Jul;46(7):580-4.

[The initial investigation of the expression of glycosyltransferases in the retina of streptomycin diabetic rats].

[Article in Chinese]

Author information

1
Department of Ophthalmology, The First People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China.

Abstract

OBJECTIVE:

To investigate whether hyperglycemia affect the expression of glycosyltransferases and Enzyme-catalyzed glycosylation in the retina of diabetic rat.

METHOD:

It was an experimental study. RT-PCR was used to analyze the mRNA level of six glycosyltransferases in the retina of streptozocin diabetic rats; Lectin blot assay with RCA-I was performed to investigate the level of Galβ 1→4GlcNAc or N-glycans on total retinal glycoproteins.

RESULTS:

mRNA level of six glycosyltransferases in the retina of streptomycin diabetic rats and of normal rat is: O-linked N-acetylglucosamine transferase (0.1650 ± 0.1160 versus 0.1160 ± 0.036); UDP-Gal: betaGlcNAc beta1,3-galactosyltransferase (0.0186 ± 0.0122 versus 0.0152 ± 0.0047); alpha 1,4-galactosyltransferase (0.0040 ± 0.0040 versus 0.0054 ± 0.0022); mannoside acetylglucosaminyltransferase 1 (0.0228 ± 0.0166 versus 0.0187 ± 0.0050); UDP-glucose ceramide glucosyltransferase (0.0129 ± 0.0096 versus 0.0116 ± 0.0040); UDP-Gal: betaGlcNAc beta 1,4-galactosyltransferase 1 (0.0157 ± 0.0010 versus 0.0081 ± 0.0016). The mRNA level of UDP-Gal: betaGlcNAc beta 1,4-galactosyltransferase 1 was up-regulated in the retina of streptomycin diabetic rats (t = 6.847, P = 0.002). Consistent with this, the level of Galβ 1→4GlcNAc of glycoproteins in streptomycin diabetic rat's retina was strengthened compared with that in control rats.

CONCLUSIONS:

The results from this study showed that Hyperglycemia could up-regulate the expression of UDP-Gal: betaGlcNAc beta 1,4-galactosyltransferase 1 and strengthen the level of Galβ 1→4GlcNAc of glycoproteins in the retina of streptomycin diabetic rats. Our initial results will contribute to the research for the relation between the Enzyme-catalyzed Glycosylation and the etiopathogenesis of diabetic retinopathy.

PMID:
21054963
[Indexed for MEDLINE]
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