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Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2157-60.

[Simultaneous determination of multicomponent in Xuefuzhuyu granule with different UV-wavelength by HPLC].

[Article in Chinese]

Author information

1
Tianjin High-Standard Quality Testing Lab., Tianjin 300081, China. liuzhenghui_2008@163.com

Abstract

OBJECTIVE:

To establish the method for the quality control of Xuefuzhuyu granule.

METHOD:

The content of Semen Armeniacae Amarum PE, Hydroxysafflor yellow A, paeoniflorin and ferulic acid in Xuefuzhuyu granule were determined by HPLC under multi-wavelength of 210, 403, 230, 316 nm.

RESULT:

The calibration curve of Semen Armeniacae Amarum PE. hydroxysafflor yellow A, paeoniflorin and ferulic acid were linear within 0.1154-0.9232 (r = 0.9998), 0.0219-0.1754 (r = 0.9994), 0.3952-3.1616 (r = 0.9995), and 0.0426-0.3408 microg (r = 0.9998), respectively. The average recoveries were 101.78% (RSD was 1.7%), 99.60% (RSD was 2.9%), 98.90% (RSD was 2.0%), and 100.31% (RSD was 1.8%), respectively.

CONCLUSION:

The method of quantification is accurate, rapid and reliable for the quality control of Xuefuzhuyu granule.

PMID:
21046752
[Indexed for MEDLINE]

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