Format

Send to

Choose Destination
See comment in PubMed Commons below
Fish Shellfish Immunol. 2011 Nov;31(5):688-96. doi: 10.1016/j.fsi.2010.10.018. Epub 2010 Oct 31.

Antarctic teleost immunoglobulins: more extreme, more interesting.

Author information

1
Institute of Protein Biochemistry, CNR, Via P. Castellino 111, 80131 Naples, Italy. mr.coscia@ibp.cnr.it

Abstract

We have investigated the immunoglobulin molecule and the genes encoding it in teleosts living in the Antarctic seas at the constant temperature of -1.86 °C. The majority of Antarctic teleosts belong to the suborder Notothenioidei (Perciformes), which includes only a few non-Antarctic species. Twenty-one Antarctic and two non-Antarctic Notothenioid species were included in our studies. We sequenced immunoglobulin light chains in two species and μ heavy chains, partially or totally, in twenty species. In the case of heavy chain, genomic DNA and the cDNA encoding the secreted and the membrane form were analyzed. From one species, Trematomus bernacchii, a spleen cDNA library was constructed to evaluate the diversity of VH gene segments. T. bernacchii IgM, purified from the serum and bile, was characterized. Homology Modelling and Molecular Dynamics were used to determine the molecular structure of T. bernacchii and Chionodraco hamatus immunoglobulin domains. This paper sums up the previous results and broadens them with the addition of unpublished data.

PMID:
21044686
DOI:
10.1016/j.fsi.2010.10.018
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center