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Cytokine. 1990 Nov;2(6):464-9.

Tumor necrosis factor alpha induction in human monocytes.

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1
Byelorussian Institute of Hematology and Blood Transfusion, Minsk, USSR.

Abstract

The present study was undertaken to assess the presence of tumor necrosis factor (TNF)-alpha mRNA and protein in circulating human blood monocytes and to study the TNF-alpha gene expression in human monocytes isolated by continuous Percoll gradient fractionation. The technique of RNA isolation directly from the blood samples was used to study TNF-alpha mRNA expression in circulating human blood leukocytes. It was shown that human blood leukocytes of healthy donors contained no presynthesized pool of TNF-alpha mRNA as well as no TNF-alpha protein. It was found that early pretreatment with cycloheximide interferes with TNF-alpha mRNA induction by Staphylococcus aureus.

PMID:
2104238
[Indexed for MEDLINE]
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