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J Am Chem Soc. 2010 Nov 24;132(46):16352-3. doi: 10.1021/ja107492b. Epub 2010 Nov 1.

Discrete structure of an RNA folding intermediate revealed by cryo-electron microscopy.

Author information

1
Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, United States.

Abstract

RNA folding occurs via a series of transitions between metastable intermediate states. It is unknown whether folding intermediates are discrete structures folding along defined pathways or heterogeneous ensembles folding along broad landscapes. We use cryo-electron microscopy and single-particle image reconstruction to determine the structure of the major folding intermediate of the specificity domain of a ribonuclease P ribozyme. Our results support the existence of a discrete conformation for this folding intermediate.

PMID:
21038867
PMCID:
PMC2988076
DOI:
10.1021/ja107492b
[Indexed for MEDLINE]
Free PMC Article

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