[Effects of Chinese herbal compound for supplementing qi and activating blood circulation on actin, Cx43 expressions and gap junctional intercellular communication functions of myocardial cells in patients with Coxsackie virus B 3 viral myocarditis]

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Aug;30(8):880-3.
[Article in Chinese]

Abstract

Objective: To observe the effect of Chinese herbal compound for supplementing qi and activating blood circulation (CHC) on the gap junctional intercellular communication (GJIC) function of myocardial cells in patients with Coxsackie virus B 3 (CVB3) viral myocarditis.

Methods: Expressions of actin and connexin43 (Cx43) in myocardial cells of patients arranged in three groups (the normal control group, the viral infected group and the CHC treated group) were detected by immunohistochemical method; the fluorescence photobleaching recovery rate of cells was detected by laser scanning confocal microscope.

Results: As compared with the viral infected group, the expressions of actin and Cx43 were increased and the GJIC function was improved in the CHC treated group.

Conclusion: CHC could antagonize viral injury on skeleton protein, and repair the structure of gap junction channel to improve the GJIC function of myocardial cells after being attacked by CVB3.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics
  • Actins / metabolism*
  • Animals
  • Animals, Newborn
  • Cell Communication / physiology
  • Connexin 43 / genetics
  • Connexin 43 / metabolism*
  • Coxsackievirus Infections / metabolism*
  • Drugs, Chinese Herbal / pharmacology*
  • Enterovirus B, Human
  • Gap Junctions / physiology
  • Myocarditis / metabolism
  • Myocarditis / virology*
  • Myocardium / metabolism
  • Rats
  • Rats, Wistar

Substances

  • Actins
  • Connexin 43
  • Drugs, Chinese Herbal