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Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524. Epub 2010 Oct 31.

Rapid blue-light-mediated induction of protein interactions in living cells.

Author information

1
Department of Neurobiology, Duke University Medical Center, Durham North Carolina, USA.

Abstract

Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate the utility of this system by inducing protein translocation, transcription and Cre recombinase-mediated DNA recombination using light.

PMID:
21037589
PMCID:
PMC3059133
DOI:
10.1038/nmeth.1524
[Indexed for MEDLINE]
Free PMC Article

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