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Am J Trop Med Hyg. 2010 Nov;83(5):1146-55. doi: 10.4269/ajtmh.2010.10-0212.

Evaluation of three commercially available Japanese encephalitis virus IgM enzyme-linked immunosorbent assays.

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  • 1Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Fort Collins, Colorado, USA. fxa3@cdc.gov

Abstract

We evaluated performance of three commercial Japanese encephalitis virus (JEV) IgM antibody capture enzyme-linked immunosorbent assay (MAC ELISA) kits with a panel of serological specimens collected during a surveillance project of acute encephalitis syndrome in India and acute meningitis and encephalitis syndrome in Bangladesh. The serum and cerebral spinal fluid specimens had been referred to the Centers for Disease Control and Prevention (CDC) for confirmatory testing. The CDC results and specimen classifications were considered the reference standard. All three commercial kits had high specificity (95-99.5%), but low sensitivities, ranging from 17-57%, with both serum and cerebrospinal fluid samples. Specific factors contributing to low sensitivity compared with the CDC ELISA could not be determined through further analysis of the limits and dilution end points of IgM detection.

PMID:
21036854
PMCID:
PMC2963986
DOI:
10.4269/ajtmh.2010.10-0212
[PubMed - indexed for MEDLINE]
Free PMC Article
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