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Curr Opin Chem Biol. 2011 Feb;15(1):32-7. doi: 10.1016/j.cbpa.2010.10.006. Epub 2010 Oct 29.

Chemical methods to detect S-nitrosation.

Author information

1
Department of Chemistry, Washington State University, Pullman, WA 99164, USA.

Abstract

Nitric oxide (NO) is a cell-signaling molecule involved in a number of physiological and pathophysiological processes. Modification of cysteine residues by NO (or NO metabolites), that is S-nitrosation, changes the function of a broad spectrum of proteins. This reaction represents an important post-translational modification that transduces NO-dependent signals. However, the detection and quantification of S-nitrosation in biological samples remain a challenge mainly because of the lability of S-nitrosation products: S-nitrosothiols (SNO). In this review we summarize recent developments of the methods to detect S-nitrosation. Our focus is on the methods which can be used to directly conjugate the site(s) of S-nitrosation.

PMID:
21036657
PMCID:
PMC3033964
DOI:
10.1016/j.cbpa.2010.10.006
[Indexed for MEDLINE]
Free PMC Article

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