Format

Send to

Choose Destination
Plant Mol Biol. 1990 Dec;15(6):941-6.

Structure and expression of a tobacco beta-1,3-glucanase gene.

Author information

1
Laboratory of Plant Molecular Biology, Fermentation Research Institute, Ibaraki, Japan.

Abstract

We determined the primary structure of a tobacco beta-1,3-glucanase gene. The beta-1,3-glucanase gene has a single large intron, and the intron separates coding regions of the signal peptide and the mature enzyme. Analysis of the 5'-flanking region sequence revealed an 11 bp GC-rich element with perfect homology to the putative regulatory sequence of tobacco chitinase genes. RNA blot analysis showed that levels of mRNAs of beta-1,3-glucanase and chitinase are coordinately increased in response to ethylene and salicylic acid. Accumulation of beta-1,3-glucanase mRNA in suspension-cultured cells is rapidly induced at late logarithmic growth phase. Members of the tobacco beta-1,3-glucanase gene families are classified into two subfamilies. One of the subfamilies appeared to be transcriptionally inactive.

PMID:
2103484
DOI:
10.1007/bf00039434
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center