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Biochimie. 2011 Mar;93(3):399-408. doi: 10.1016/j.biochi.2010.10.011. Epub 2010 Oct 27.

Effects of 8-methylguanine on structure, stability and kinetics of formation of tetramolecular quadruplexes.

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1
INSERM U869, European Institute of Chemistry and Biology, Bordeaux University, 2 rue Robert Escarpit, Pessac, France.

Abstract

Tetramolecular G-quadruplexes result from the association of four guanine-rich strands. Modification of the backbone strand or the guanine bases of the oligonucleotide may improve stability or introduce new functionalities. In this regard, the 8 position of a guanosine is particularly suitable for introduction of modifications since as it is positioned in the groove of the quadruplex structure. Modifications at this position should not interfere with structural assembly as would changes at Watson-Crick and Hoogsteen sites. In this study, we investigated the effect of an 8-methyl-2'-deoxyguanosine residue (M) on the structure and stability of tetramolecular parallel G-quadruplexes. In some cases, the presence of this residue resulted in the formation of unusual quadruplex structures containing all-syn tetrads. Furthermore, the modified nucleoside M at the 5'-end of the sequence accelerated quadruplex formation by 15-fold or more relative to the unmodified oligonucleotide, which makes this nucleobase an attractive replacement for guanine in the context of tetramolecular parallel quadruplexes.

PMID:
21034790
DOI:
10.1016/j.biochi.2010.10.011
[Indexed for MEDLINE]
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