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Rev Sci Instrum. 2010 Oct;81(10):105102. doi: 10.1063/1.3481164.

NMR probe for pressure-jump experiments up to 250 bars and 3 ms jump time.

Author information

1
Insitut für Physik, Martin-Luther University Halle-Wittenberg, Betty-Heimann-Str. 7, D-06120 Halle/Saale, Germany.

Abstract

We describe the design and performance of a pressure-jump instrument for time-resolved NMR experiments. Initial pressure of up to 250 bars can be produced by means of a HPLC pump and distilled water as a pressure-transmitting liquid. Fast pressure release at a time resolution of 3 ms is achieved using a fast acting valve driven by a piezostack close to the sample chamber. The pressure-jump cell is placed together with two valves in an especially designed NMR probe, which can be used in standard spectrometers with wide-bore magnets. All functions of the instrument are personal computer controlled. The equipment is designed for investigations on systems of biological interest, especially lipid-water dispersions. A theoretical consideration implies that probably the limited speed of valve opening determines the lower boundary of the jump time. The performance is illustrated by time-resolved NMR spectra across the phase transition of a phospholipid-water dispersion after a pressure jump from 100 bars to atmospheric pressure.

PMID:
21034114
DOI:
10.1063/1.3481164
[Indexed for MEDLINE]

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