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Nat Protoc. 2010 Nov;5(11):1775-1785. doi: 10.1038/nprot.2010.134. Epub 2010 Oct 14.

An efficient method of directly cloning chimpanzee adenovirus as a vaccine vector.

Author information

1
The Wistar Institute, Philadelphia, Pennsylvania, USA.
#
Contributed equally

Abstract

Adenoviral vectors have shown great promise as vaccine carriers and in gene transfer to correct underlying genetic diseases. Traditionally, construction of adenoviral vectors is complex and time consuming. In this paper, we provide an improved method for efficient generation of novel adenoviral vectors by using direct cloning. We introduce a feasible and detailed protocol for the development of chimpanzee adenoviruses (Ads) as molecular clones, as well as for the generation of recombinant virus from the molecular clones. Recombinant viruses are genetically stable and induce potent immune responses in animals. Generation of new Ad molecular clones or new recombinant Ad can be achieved in 2 months or 2 weeks, respectively.

PMID:
21030953
PMCID:
PMC3994680
DOI:
10.1038/nprot.2010.134
[Indexed for MEDLINE]
Free PMC Article

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