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Nat Commun. 2010 Oct 19;1:100. doi: 10.1038/ncomms1093.

Protein-binding assays in biological liquids using microscale thermophoresis.

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1
Department of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universit√§t M√ľnchen, Amalienstrasse 54, Munich 80799, Germany.

Abstract

Protein interactions inside the human body are expected to differ from the situation in vitro. This is crucial when investigating protein functions or developing new drugs. In this study, we present a sample-efficient, free-solution method, termed microscale thermophoresis, that is capable of analysing interactions of proteins or small molecules in biological liquids such as blood serum or cell lysate. The technique is based on the thermophoresis of molecules, which provides information about molecule size, charge and hydration shell. We validated the method using immunologically relevant systems including human interferon gamma and the interaction of calmodulin with calcium. The affinity of the small-molecule inhibitor quercetin to its kinase PKA was determined in buffer and human serum, revealing a 400-fold reduced affinity in serum. This information about the influence of the biological matrix may allow to make more reliable conclusions on protein functionality, and may facilitate more efficient drug development.

PMID:
20981028
DOI:
10.1038/ncomms1093
[Indexed for MEDLINE]
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