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J Infect Dis. 2010 Dec 1;202(11):1659-66. doi: 10.1086/657158. Epub 2010 Oct 27.

Circulating antibody-secreting cells during acute respiratory syncytial virus infection in adults.

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Division of Pulmonary and Critical Care Medicine, University of Rochester and Rochester General Hospital, Rochester, New York, USA.



The specificity and duration of circulating human antibody-secreting cells (ASCs) after vaccination have been well described, but characteristics of ASCs during acute respiratory infections have not been well studied.


Circulating antigen-specific ASCs were measured at 3 time points (enrollment, days 10-16, and days 22-45) in 40 adults during respiratory syncytial virus (RSV) infection.


Of the 40 patients, 36 (90%) had detectable circulating RSV F protein-specific ASCs within 11 days after illness onset. The magnitude of the RSV-specific ASCs was 1-1500 spots per 10⁶ peripheral blood mononuclear cells (mean frequency [± standard deviation], 200 ± 256 spots per 10⁶ peripheral blood mononuclear cells). ASCs were detected on day 8-16 and day 22-45 after symptom onset in 78% and 48% of subjects, respectively. Subjects shedding virus for >10 days were more likely to have a positive response to ASC enzyme-linked immunospot assay at the late time point than those shedding for ≤10 days (8 of 12 subjects vs 2 of 11 subjects; P = .02).


The kinetics of ASC circulation during acute mucosal viral infections was more prolonged than that we had observed after a single intramuscular injection with inactivated influenza vaccine in a study reported elsewhere. The association between the duration of virus shedding and the persistence of detectable viral-specific ASCs suggests that ongoing antigen persistence induces a prolonged temporal pattern of ASC generation.

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