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J Exp Med. 2010 Nov 22;207(12):2621-30. doi: 10.1084/jem.20100864. Epub 2010 Oct 25.

IkappaBbeta is an essential co-activator for LPS-induced IL-1beta transcription in vivo.

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1
II. Medizinische Klinik, Technische Universität München, 81675 München, Germany.

Abstract

Inhibitor of κB (IκB) β (IκBβ) represents one of the major primary regulators of NF-κB in mammals. In contrast to the defined regulatory interplay between NF-κB and IκBα, much less is known about the biological function of IκBβ. To elucidate the physiological role of IκBβ in NF-κB signaling in vivo, we generated IκBβ-deficient mice. These animals proved to be highly refractory to LPS-induced lethality, accompanied by a strong reduction in sepsis-associated cytokine production. In response to LPS, IκBβ is recruited to the IL-1β promoter forming a complex with the NF-κB subunits RelA/c-Rel required for IL-1β transcription. Further transcriptome analysis of LPS-stimulated wild-type and IκBβ-deficient BM-derived macrophages revealed several other genes with known regulatory functions in innate immunity arguing that a subset of NF-κB target genes is under control of IκBβ. Collectively, these findings provide an essential proinflammatory role for IκBβ in vivo, and establish a critical function for IκBβ as a transcriptional coactivator under inflammatory conditions.

PMID:
20975042
PMCID:
PMC2989768
DOI:
10.1084/jem.20100864
[Indexed for MEDLINE]
Free PMC Article
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